California NanoSystems Institute
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Fuyuhiko Tamanoi, Ph.D.

Professor, Microbiology, Immunology & Molecular Genetics
Director, JCCC Signal Transduction and Therapeutics Program Area

Ph.D., Nagoya University, 1977
M.S., Tokyo University, Tokyo, Japan, 1972 - 1974
B.S., Tokyo University, Tokyo, Japan, 1968 - 1972

Professional Societies:
American Association for Cancer Research
American Society for Biochemistry & Molecular Biology

Academic Experience:
Academic Experience:
1977 - 1980 Harvard Medical School, Boston, Massachusetts, Biological Chemistry

Contact Information:
Work Email Address:
Laboratory Address: Tamanoi Laboratory
Molecular Science Building 2711, 2715
Los Angeles, CA 90095
Office Address: 405 Hilgard Avenue
Los Angeles, CA 90095
Work Address: UCLA MIMG
Molecular Science Building 2610A
BOX 951489
Los Angeles, CA 90095
ARR Papers:
Home Page:
Fax Number: 310-206-5231 Fax
Work Phone Number: 310-206-7318 Phone
310-825-7754 Work
Research Interests:

Identification of novel anti-cancer drugs and their nanodelivery
Recent advance in the study of the molecular basis of diseases led to the development of a new class of drugs that are mechanism based. We are currently generating novel anti-cancer drugs that are aimed at blocking lipid modification of membrane-bound molecular switches. These drugs are packaged into nanoparticles to achieve nanodelivery into cancer cells.

Technical Research Interest:

Signal Transduction Inhibitors and Their Nanodelivery
Professor Tamanoi's group is interested in molecular switches and cellular signaling networks. The research focuses on the Ras family proteins that act as a nanoscale switch by shuttling between a GTP bound active form and a GDP-bound inactive form. Current research is aimed at characterizing two members of the Ras family proteins called Ras and Rheb. While Ras activates signaling pathways such as Raf/MAP kinase signaling, Rheb activates mTOR signaling that regulates protein synthesis. Overactivation of these molecular switches forms the basis of human diseases such as cancer, neurofibromatosis and tuberous sclerosis. Another research focus is to carry out nanoscale modulation of the signaling network. To sccomplish this, small organic molecule compounds that can regulate the activity of molecular switches have been identified. One type of compound blocks their lipid modification resulting in the inhibition of their membrane association. Experiments are underway to package these compounds into nanoparticles and achieve nanodelivery into human cancer cells.

Selected Publications:

Akhavan David, Pourzia Alexandra L, Nourian Alex A, Williams Kevin J, Nathanson David, Babic Ivan, Villa Genaro R, Tanaka Kazuhiro, Nael Ali, Yang Huijun, Dang Julie, Vinters Harry V, Yong William H, Flagg Mitchell, Tamanoi Fuyuhiko, Sasayama Takashi, James C David, Kornblum Harley I, Cloughesy Tim F, Cavenee Webster K, Bensinger Steven J, Mischel Paul S, De-Repression of PDGFRβ Transcription Promotes Acquired Resistance to EGFR Tyrosine Kinase Inhibitors in Glioblastoma Patients, Cancer discovery, 2013, 3 (5), 534-547.
Zimonjic Drazen B, Chan Lai N, Tripathi Veenu, Lu Jie, Kwon Ohyun, Popescu Nicholas C, Lowy Douglas R, Tamanoi Fuyuhiko, In vitro and in vivo effects of geranylgeranyltransferase I inhibitor P61A6 on non-small cell lung cancer cells, BMC cancer, 2013, 13 (5), 198.
Yanes Rolando E, Tarn Derrick, Hwang Angela A, Ferris Daniel P, Sherman Sean P, Thomas Courtney R, Lu Jie, Pyle April D, Zink Jeffrey I, Tamanoi Fuyuhiko, Involvement of lysosomal exocytosis in the excretion of mesoporous silica nanoparticles and enhancement of the drug delivery effect by exocytosis inhibition, Small (Weinheim an der Bergstrasse, Germany), 2013, 9 (5), 697-704.
Lau Yuen A, Henderson Bryana L, Lu Jie, Ferris Daniel P, Tamanoi Fuyuhiko, Zink Jeffrey I, Continuous spectroscopic measurements of photo-stimulated release of molecules by nanomachines in a single living cell, Nanoscale, 2012, 4 (11), 3482-9.
Yanes Rolando E, Tamanoi Fuyuhiko, Development of mesoporous silica nanomaterials as a vehicle for anticancer drug delivery, Therapeutic delivery, 2012, 3 (3), 389-404.
Rettig Matthew, Trinidad Kenny, Pezeshkpour G, Frost Patrick, Sharma Sherven, Moatamed Farhad, Tamanoi Fuyuhiko, Mortazavi Fariborz, PAK1 kinase promotes cell motility and invasiveness through CRK-II serine phosphorylation in non-small cell lung cancer cells, PloS one, 2012, 7 (7), e42012.
Nakashima Akio, Otsubo Yoko, Yamashita Akira, Sato Tatsuhiro, Yamamoto Masayuki, Tamanoi Fuyuhiko, Psk1, an AGC kinase family member in fission yeast, is directly phosphorylated and controlled by TORC1 and functions as S6 kinase, Journal of cell science, 2012, 125 (Pt 23), 5840-9.
Hon Nick K, Shaposhnik Zory, Diebold Eric D, Tamanoi Fuyuhiko, Jalali Bahram, Tailoring the biodegradability of porous silicon nanoparticles, Journal of biomedical materials research. Part A, 2012, 100 (12), 3416-21.
Hardt Molly, Chantaravisoot Naphat, Tamanoi Fuyuhiko, Activating mutations of TOR (target of rapamycin), Genes to cells : devoted to molecular & cellular mechanisms, 2011, 16 (2), 141-51.
Chan Lai N, Fiji Hannah D G, Watanabe Masaru, Kwon Ohyun, Tamanoi Fuyuhiko, Identification and characterization of mechanism of action of P61-E7, a novel phosphine catalysis-based inhibitor of geranylgeranyltransferase-I, PloS one, 2011, 6 (10), e26135.
Lu Jie, Li Zongxi, Zink Jeffrey I, Tamanoi Fuyuhiko, In vivo tumor suppression efficacy of mesoporous silica nanoparticles-based drug-delivery system: enhanced efficacy by folate modification, Nanomedicine : nanotechnology, biology, and medicine, 2011, 133 (23).
Tamanoi Fuyuhiko, Ras signaling in yeast, Genes & cancer, 2011, 2 (3), 210-5.
Ferris Daniel P, Lu Jie, Gothard Chris, Yanes Rolando, Thomas Courtney R, Olsen John-Carl, Stoddart J Fraser, Tamanoi Fuyuhiko, Zink Jeffrey I, Synthesis of biomolecule-modified mesoporous silica nanoparticles for targeted hydrophobic drug delivery to cancer cells, Small (Weinheim an der Bergstrasse, Germany), 2011, 7 (13), 1816-26.
Xue Min, Zhong Xing, Shaposhnik Zory, Qu Yongquan, Tamanoi Fuyuhiko, Duan Xiangfeng, Zink Jeffrey I, pH-Operated mechanized porous silicon nanoparticles, Journal of the American Chemical Society, 2011, 133 (23), 8798-801.
Meng Huan, Xue Min, Xia Tian, Zhao Yan-Li, Tamanoi Fuyuhiko, Stoddart J Fraser, Zink Jeffrey I, Nel Andre E, Autonomous in vitro anticancer drug release from mesoporous silica nanoparticles by pH-sensitive nanovalves, Journal of the American Chemical Society, 2010, 132 (36), 12690-7.
Lu Jie, Liong Monty, Li Zongxi, Zink Jeffrey I, Tamanoi Fuyuhiko, Biocompatibility, biodistribution, and drug-delivery efficiency of mesoporous silica nanoparticles for cancer therapy in animals, Small (Weinheim an der Bergstrasse, Germany), 2010, 6 (16), 1794-805.
A B Hanker, N Mitin, R S Wilder, E P Henske, F Tamanoi, A D Cox and C J Der, Differential requirement of CAAX-mediated posttranslational processing for Rheb localization and signaling, Oncogene, 2010, 29, 380–391.
Nakashima Akio, Sato Tatsuhiro, Tamanoi Fuyuhiko, Fission yeast TORC1 regulates phosphorylation of ribosomal S6 proteins in response to nutrients and its activity is inhibited by rapamycin, Journal of cell science, 2010, 123 (Pt 5), 777-86.
Hom Christopher, Lu Jie, Liong Monty, Luo Hanzhi, Li Zongxi, Zink Jeffrey I, Tamanoi Fuyuhiko, Mesoporous silica nanoparticles facilitate delivery of siRNA to shutdown signaling pathways in mammalian cells, Small (Weinheim an der Bergstrasse, Germany), 2010, 6 (11), 1185-90.
Jiang Huaidong, Song Changyong, Chen Chien-Chun, Xu Rui, Raines Kevin S, Fahimian Benjamin P, Lu Chien-Hung, Lee Ting-Kuo, Nakashima Akio, Urano Jun, Ishikawa Tetsuya, Tamanoi Fuyuhiko, Miao Jianwei, Quantitative 3D imaging of whole, unstained cells by using X-ray diffraction microscopy, Proceedings of the National Academy of Sciences of the United States of America, 2010, 107 (25), 11234-9.
Sato Tatsuhiro, Nakashima Akio, Tamanoi Fuyuhiko, [Rheb-mTOR signaling pathway involved in tumor formation], Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme, 2010, 55 (1), 11-7.
Chan Lai N, Hart Courtenay, Guo Lea, Nyberg Tamara, Davies Brandon S J, Fong Loren G, Young Stephen G, Agnew Brian J, Tamanoi Fuyuhiko, A novel approach to tag and identify geranylgeranylated proteins, Electrophoresis, 2009, 30 (20), 3598-606.
Kugawa, F. Suzuki, T. Miyata, M. Tomono, K. Tamanoi, F. , Construction of a model cell line for the assay of MDR1 (Multi Drug Resistance gene-1) substrates/inhibitors using HeLa cells, Pharmazie, 2009, 64 (5), 296-300.
Lu Jie, Chan Lai, Fiji Hannah D G, Dahl Russell, Kwon Ohyun, Tamanoi Fuyuhiko, In vivo antitumor effect of a novel inhibitor of protein geranylgeranyltransferase-I, Molecular cancer therapeutics, 2009, 8 (5), 1218-26.
Davies Brandon S J, Yang Shao H, Farber Emily, Lee Roger, Buck Suzanne B, Andres Douglas A, Spielmann H Peter, Agnew Brian J, Tamanoi Fuyuhiko, Fong Loren G, Young Stephen G, Increasing the length of progerin's isoprenyl anchor does not worsen bone disease or survival in mice with Hutchinson-Gilford progeria syndrome, Journal of lipid research, 2009, 50 (1), 126-34.
Klichko Yaroslav, Liong Monty, Choi Eunshil, Angelos Sarah, Nel Andre E, Stoddart J Fraser, Tamanoi Fuyuhiko, Zink Jeffrey I, Mesostructured Silica for Optical Functionality, Nanomachines, and Drug Delivery, Journal of the American Ceramic Society. American Ceramic Society, 2009, 92 (s1), s2-s10.
Hom Christopher, Lu Jie, Tamanoi Fuyuhiko, Silica nanoparticles as a delivery system for nucleic acid-based reagents, Journal of materials chemistry, 2009, 19 (35), 6308-6316.
Sato Tatsuhiro, Nakashima Akio, Guo Lea, Tamanoi Fuyuhiko, Specific activation of mTORC1 by Rheb G-protein in vitro involves enhanced recruitment of its substrate protein, The Journal of biological chemistry, 2009, 284 (19), 12783-91.
Zhou Jun, Vos Chantal Chanel, Gjyrezi Ada, Yoshida Minoru, Khuri Fadlo R, Tamanoi Fuyuhiko, Giannakakou Paraskevi, The protein farnesyltransferase regulates HDAC6 activity in a microtubule-dependent manner, The Journal of biological chemistry, 2009, 284 (15), 9648-55.
Short John D, Houston Kevin D, Dere Ruhee, Cai Sheng-Li, Kim Jinhee, Johnson Charles L, Broaddus Russell R, Shen Jianjun, Miyamoto Susie, Tamanoi Fuyuhiko, Kwiatkowski David, Mills Gordon B, Walker Cheryl Lyn, AMP-activated protein kinase signaling results in cytoplasmic sequestration of p27, Cancer research, 2008, 68 (16), 6496-506.
Sato Tatsuhiro, Umetsu Akiko, Tamanoi Fuyuhiko, Characterization of the Rheb-mTOR signaling pathway in mammalian cells: constitutive active mutants of Rheb and mTOR, Methods in enzymology, 2008, 438 (3), 307-20.
Ikeda Kyoko, Morigasaki Susumu, Tatebe Hisashi, Tamanoi Fuyuhiko, Shiozaki Kazuhiro, Fission yeast TOR complex 2 activates the AGC-family Gad8 kinase essential for stress resistance and cell cycle control, Cell cycle (Georgetown, Tex.), 2008, 7 (3), 358-64.
Watanabe Masaru, Fiji Hannah D G, Guo Lea, Chan Lai, Kinderman Sape S, Slamon Dennis J, Kwon Ohyun, Tamanoi Fuyuhiko, Inhibitors of protein geranylgeranyltransferase I and Rab geranylgeranyltransferase identified from a library of allenoate-derived compounds, The Journal of biological chemistry, 2008, 283 (15), 9571-9.
Lu Jie, Choi Eunshil, Tamanoi Fuyuhiko, Zink Jeffrey I, Light-activated nanoimpeller-controlled drug release in cancer cells, Small (Weinheim an der Bergstrasse, Germany), 2008, 4 (4), 421-6.
Liong Monty, Lu Jie, Kovochich Michael, Xia Tian, Ruehm Stefan G, Nel Andre E, Tamanoi Fuyuhiko, Zink Jeffrey I, Multifunctional inorganic nanoparticles for imaging, targeting, and drug delivery, ACS nano, 2008, 2 (5), 889-96.
Aspuria Paul-Joseph, Tamanoi Fuyuhiko, The Tsc/Rheb signaling pathway controls basic amino acid uptake via the Cat1 permease in fission yeast, Molecular genetics and genomics : MGG, 2008, 279 (5), 441-50.
Matsuo Tomohiko, Otsubo Yoko, Urano Jun, Tamanoi Fuyuhiko, Yamamoto Masayuki, Loss of the TOR kinase Tor2 mimics nitrogen starvation and activates the sexual development pathway in fission yeast, Molecular and cellular biology, 2007, 27 (8), 3154-64.
Lu Jie, Liong Monty, Sherman Sean, Xia Tian, Kovochich Michael, Nel Andre E, Zink Jeffrey I, Tamanoi Fuyuhiko, Mesoporous Silica Nanoparticles for Cancer Therapy: Energy-Dependent Cellular Uptake and Delivery of Paclitaxel to Cancer Cells, Nanobiotechnology : the journal at the intersection of nanotechnology, molecular biology, and biomedical sciences, 2007, 3 (2), 89-95.
Lu Jie, Liong Monty, Zink Jeffrey I, Tamanoi Fuyuhiko, Mesoporous silica nanoparticles as a delivery system for hydrophobic anticancer drugs, Small (Weinheim an der Bergstrasse, Germany), 2007, 3 (8), 1341-6.
Urano Jun, Sato Tatsuhiro, Matsuo Tomohiko, Otsubo Yoko, Yamamoto Masayuki, Tamanoi Fuyuhiko, Point mutations in TOR confer Rheb-independent growth in fission yeast and nutrient-independent mammalian TOR signaling in mammalian cells, Proceedings of the National Academy of Sciences of the United States of America, 2007, 104 (9), 3514-9.
Castellano Sabrina, Fiji Hannah D G, Kinderman Sape S, Watanabe Masaru, Leon Pablo de, Tamanoi Fuyuhiko, Kwon Ohyun, Small-molecule inhibitors of protein geranylgeranyltransferase type I, Journal of the American Chemical Society, 2007, 129 (18), 5843-5.
Yu Fuqu, Harada Josephine N, Brown Helen J, Deng Hongyu, Song Moon Jung, Wu Ting-Ting, Kato-Stankiewicz Juran, Nelson Christian G, Vieira Jeffrey, Tamanoi Fuyuhiko, Chanda Sumit K, Sun Ren, Systematic identification of cellular signals reactivating Kaposi sarcoma-associated herpesvirus, PLoS pathogens, 2007, 3 (3), e44.
Aspuria Paul-Joseph, Sato Tatsuhiro, Tamanoi Fuyuhiko, The TSC/Rheb/TOR signaling pathway in fission yeast and mammalian cells: temperature sensitive and constitutive active mutants of TOR, Cell cycle (Georgetown, Tex.), 2007, 6 (14), 1692-5.
Patel Parthive H, Tamanoi Fuyuhiko, Increased Rheb-TOR signaling enhances sensitivity of the whole organism to oxidative stress, Journal of cell science, 2006, 119 (Pt 20), 4285-92.
Ikeda, A. Shankar, D. B. Watanabe, M. Tamanoi, F. Moore, T. B. Sakamoto, K. M. , Molecular targets and the treatment of myeloid leukemia, Molecular Genetics and Metabolism, 2006, 88 (3), 216-224.
A.P. Tabancay Jr., M.J. Comiso and F. Tamanoi, RAS family G-proteins in Saccharomyces cerevisiae and Schizosaccharomyces pombe, RAS family GTPases, 2006, 4, 277-256.
Gelb Michael H, Brunsveld Lucas, Hrycyna Christine A, Michaelis Susan, Tamanoi Fuyuhiko, Van Voorhis Wesley C, Waldmann Herbert, Therapeutic intervention based on protein prenylation and associated modifications, Nature chemical biology, 2006, 2 (10), 518-28.
Patel Parthive H, Tamanoi Fuyuhiko, Using Drosophila and yeast genetics to investigate a role for the Rheb GTPase in cell growth, Methods in enzymology, 2006, 407 (10), 443-54.
Khazak, V. Kato-Stankiewicz, J., Tamanoi, F, and Golemis, E.A. , Yeast screens for inhibitors of Ras-Raf interaction and characterization of MCP inhibitors of Ras-Raf interaction, Methods in Enzymol, 2006, 407, 612-629.
Gau Chia-Ling, Kato-Stankiewicz Juran, Jiang Chen, Miyamoto Susie, Guo Lea, Tamanoi Fuyuhiko, Farnesyltransferase inhibitors reverse altered growth and distribution of actin filaments in Tsc-deficient cells via inhibition of both rapamycin-sensitive and -insensitive pathways, Molecular cancer therapeutics, 2005, 4 (6), 918-26.
Urano Jun, Comiso Melissa J, Guo Lea, Aspuria Paul-Joseph, Deniskin Roman, Tabancay Angel P, Kato-Stankiewicz Juran, Tamanoi Fuyuhiko, Identification of novel single amino acid changes that result in hyperactivation of the unique GTPase, Rheb, in fission yeast, Molecular microbiology, 2005, 58 (4), 1074-86.
Kho Yoonjung, Kim Sung Chan, Jiang Chen, Barma Deb, Kwon Sung Won, Cheng Jinke, Jaunbergs Janis, Weinbaum Carolyn, Tamanoi Fuyuhiko, Falck John, Zhao Yingming, A tagging-via-substrate technology for detection and proteomics of farnesylated proteins, Proceedings of the National Academy of Sciences of the United States of America, 2004, 101 (34), 12479-84.
Clarke Steven, Tamanoi Fuyuhiko, Fighting cancer by disrupting C-terminal methylation of signaling proteins, The Journal of clinical investigation, 2004, 113 (4), 513-5.
Uhlmann Erik J, Li Wen, Scheidenhelm Danielle K, Gau Chia-Ling, Tamanoi Fuyuhiko, Gutmann David H, Loss of tuberous sclerosis complex 1 (Tsc1) expression results in increased Rheb/S6K pathway signaling important for astrocyte cell size regulation, Glia, 2004, 47 (2), 180-8.
Aspuria Paul-Joseph, Tamanoi Fuyuhiko, The Rheb family of GTP-binding proteins, Cellular signalling, 2004, 16 (10), 1105-12.
Hamasaki Akiyuki, Naka Hayato, Tamanoi Fuyuhiko, Umezawa Kazuo, Otsuka Masami, A novel metal-chelating inhibitor of protein farnesyltransferase, Bioorganic & medicinal chemistry letters, 2003, 13 (9), 1523-6.
Patel Parthive H, Thapar Nitika, Guo Lea, Martinez Monica, Maris John, Gau Chia-Ling, Lengyel Judith A, Tamanoi Fuyuhiko, Drosophila Rheb GTPase is required for cell cycle progression and cell growth, Journal of cell science, 2003, 116 (Pt 17), 3601-10.
Tabancay Angel P, Gau Chia-Ling, Machado Iara M P, Uhlmann Erik J, Gutmann David H, Guo Lea, Tamanoi Fuyuhiko, Identification of dominant negative mutants of Rheb GTPase and their use to implicate the involvement of human Rheb in the activation of p70S6K, The Journal of biological chemistry, 2003, 278 (41), 39921-30.
Kato-Stankiewicz Juran, Hakimi Irina, Zhi Gang, Zhang Jie, Serebriiskii Ilya, Guo Lea, Edamatsu Hironori, Koide Hiroshi, Menon Sanjay, Eckl Robert, Sakamuri Sukumar, Lu Yingchun, Chen Quin-Zene, Agarwal Seema, Baumbach William R, Golemis Erica A, Tamanoi Fuyuhiko, Khazak Vladimir, Inhibitors of Ras/Raf-1 interaction identified by two-hybrid screening revert Ras-dependent transformation phenotypes in human cancer cells, Proceedings of the National Academy of Sciences of the United States of America, 2002, 99 (22), 14398-403.