California NanoSystems Institute
CNSI
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Kenneth Bradley, Ph.D.

   
Associate Professor, Microbiology, Immunology & Molecular Genetics

Education:
Degrees:
Ph.D., Harvard University, 2002
B.A., UC Santa Cruz, 1993

Professional Societies:
American Society of Microbiology

Contact Information:
Email Address: kbradley@microbio.ucla.edu
Laboratory Address: Laboratory
MSB 2817

UNITED STATES
Mailing Address: Mail Code: 148906
Los Angeles, CA 90095
UNITED STATES
Work Address: Office
MSB 2825

UNITED STATES
Work Address: Laboratory
Molecular Sciences Building
Los Angeles, CA 90095
UNITED STATES
Work Address: Office
Molecular Sciences Building
Los Angeles, CA 90095
UNITED STATES
Fax Number: (310) 206-5231 Fax
Work Phone Number: 310-206-7465 Office
310-206-7517 Laboratory
310-206-7465 Office
310-206-7517 Laboratory
Technical Research Interest:

The Bradley lab is interested in i) identifying host proteins that are usurped by bacterial pathogens and ii) understanding how these interactions promote virulence. Specifically, we are studying protein exotoxins produced by Bacillus anthracis (the causative agent of anthrax), Campylobacter jejuni (the most common cause of bacterial diarrhea in the U.S.), Vibrio cholera (the causative agent of cholera), and Clostridium deficile (responsible for most hospital-acquired diarrhea). To better understand these interactions, our lab utilizes a number approaches including i) proteomics, ii) chemical genetics and iii) somatic cell genetics. We are also interested in developing therapeutics and detection systems for bacterial pathogens based on nanotechnology. To this end, we collaborate with a number of labs at UCLA and nationwide.


Additional Information:

Dr. Bradley received a B.A. in Biochemistry and Molecular Biology from U.C. Santa Cruz and a Ph.D. in Microbiology and Molecular Genetics from Harvard Medical School. He has been a member of the UCLA faculty since 2002 and is currently an Associate Professor in the Department of Microbiology, Immunology & Molecular Genetics. In addition, Dr. Bradley serves as Director for the Molecular Screening Shared Resource, a high-throughput screening facility that provides robotics for multiple applications including chemical genomics.

Selected Publications:

Jung, Michael E; Chamberlain, Brian T; Ho, Chi-Lee C; Gillespie, Eugene J; Bradley, Kenneth A, Structure-Activity Relationship of Semicarbazone EGA Furnishes Photoaffinity Inhibitors of Anthrax Toxin Cellular Entry, ACS Medicinal Chemistry Letters, 2014.
Gillespie, E. J.; Ho, C. L.; Balaji, K.; Clemens, D. L.; Deng, G.; Wang, Y. E.; Elsaesser, H. J.; Tamilselvam, B.; Gargi, A.; Dixon, S. D.; France, B.; Chamberlain, B. T.; Blanke, S. R.; Cheng, G.; de la Torre, J. C.; Brooks, D. G.; Jung, M. E.; Colicelli, J.; Damoiseaux, R.; Bradley, K. A., Selective inhibitor of endosomal trafficking pathways exploited by multiple toxins and viruses, Proceedings of the National Academy of Sciences of the United States of America, 2013, 110 (50), E4904-12.
Gargi, A.; Tamilselvam, B.; Powers, B.; Prouty, M. G.; Lincecum, T.; Eshraghi, A.; Maldonado-Arocho, F. J.; Wilson, B. A.; Bradley, K. A.; Blanke, S. R., Cellular interactions of the cytolethal distending toxins from Escherichia coli and Haemophilus ducreyi, The Journal of biological chemistry, 2013, 288 (11), 7492-505.
Ivask, A.; Suarez, E.; Patel, T.; Boren, D.; Ji, Z.; Holden, P.; Telesca, D.; Damoiseaux, R.; Bradley, K. A.; Godwin, H., Genome-wide bacterial toxicity screening uncovers the mechanisms of toxicity of a cationic polystyrene nanomaterial, Environmental science & technology, 2012, 46 (4), 2398-405.
Visnyei, K.; Onodera, H.; Damoiseaux, R.; Saigusa, K.; Petrosyan, S.; De Vries, D.; Ferrari, D.; Saxe, J.; Panosyan, E. H.; Masterman-Smith, M.; Mottahedeh, J.; Bradley, K. A.; Huang, J.; Sabatti, C.; Nakano, I.; Kornblum, H. I., A molecular screening approach to identify and characterize inhibitors of glioblastoma stem cells, Molecular cancer therapeutics, 2011, 10 (10), 1818-28.
Terra, J. K.; France, B.; Cote, C. K.; Jenkins, A.; Bozue, J. A.; Welkos, S. L.; Bhargava, R.; Ho, C. L.; Mehrabian, M.; Pan, C.; Lusis, A. J.; Davis, R. C.; LeVine, S. M.; Bradley, K. A., Allelic variation on murine chromosome 11 modifies host inflammatory responses and resistance to Bacillus anthracis, PLoS pathogens, 2011, 7 (12), e1002469.
Larabee, J. L.; Maldonado-Arocho, F. J.; Pacheco, S.; France, B.; DeGiusti, K.; Shakir, S. M.; Bradley, K. A.; Ballard, J. D., Glycogen synthase kinase 3 activation is important for anthrax edema toxin-induced dendritic cell maturation and anthrax toxin receptor 2 expression in macrophages, Infection and immunity, 2011, 79 (8), 3302-8.
Kim, K.; Damoiseaux, R.; Norris, A. J.; Rivina, L.; Bradley, K.; Jung, M. E.; Gatti, R. A.; Schiestl, R. H.; McBride, W. H., High throughput screening of small molecule libraries for modifiers of radiation responses, Int J Radiat Biol, 2011.
Zhang, H.; Xia, T.; Meng, H.; Xue, M.; George, S.; Ji, Z.; Wang, X.; Liu, R.; Wang, M.; France, B.; Rallo, R.; Damoiseaux, R.; Cohen, Y.; Bradley, K. A.; Zink, J. I.; Nel, A. E., Differential Expression of Syndecan-1 Mediates Cationic Nanoparticle Toxicity in Undifferentiated versus Differentiated Normal Human Bronchial Epithelial Cells, ACS Nano, 2011.
George, S.; Xia, T.; Rallo, R.; Zhao, Y.; Ji, Z.; Lin, S.; Wang, X.; Zhang, H.; France, B.; Schoenfeld, D.; Damoiseaux, R.; Liu, R.; Bradley, K. A.; Cohen, Y.; Nel, A. E., Use of a High-Throughput Screening Approach Coupled with In Vivo Zebrafish Embryo Screening To Develop Hazard Ranking for Engineered Nanomaterials, ACS Nano, 2011.
Rallo, R.; France, B.; Liu, R.; Nair, S.; George, S.; Damoiseaux, R.; Giralt, F.; Nel, A.; Bradley, K.; Cohen, Y., Self-Organizing Map Analysis of Toxicity-Related Cell Signaling Pathways for Metal and Metal Oxide Nanoparticles, Environ Sci Technol, 2011.
Bradley, K. A.; LeVine, S. M., Anthrax toxin delivers a one-two punch, Cell Host Microbe, 2010, 8 (5), 394-5.
Ramey, J. D.; Villareal, V. A.; Ng, C.; Ward, S. C.; Xiong, J. P.; Clubb, R. T.; Bradley, K. A., Anthrax toxin receptor 1/tumor endothelial marker 8: mutation of conserved inserted domain residues overrides cytosolic control of protective antigen binding, Biochemistry, 2010, 49 (34), 7403-10.
Eshraghi, A.; Maldonado-Arocho, F. J.; Gargi, A.; Cardwell, M. M.; Prouty, M. G.; Blanke, S. R.; Bradley, K. A., Cytolethal distending toxin family members are differentially affected by alterations in host glycans and membrane cholesterol, J Biol Chem, 2010, 285 (24), 18199-207.
George, S.; Pokhrel, S.; Xia, T.; Gilbert, B.; Ji, Z.; Schowalter, M.; Rosenauer, A.; Damoiseaux, R.; Bradley, K. A.; Madler, L.; Nel, A. E., Use of a rapid cytotoxicity screening approach to engineer a safer zinc oxide nanoparticle through iron doping, ACS Nano, 2010, 4 (1), 15-29.
Terra, J. K.; Cote, C. K.; France, B.; Jenkins, A. L.; Bozue, J. A.; Welkos, S. L.; LeVine, S. M.; Bradley, K. A., Cutting edge: resistance to Bacillus anthracis infection mediated by a lethal toxin sensitive allele of Nalp1b/Nlrp1b, J Immunol, 2010, 184 (1), 17-20.
Godwin, H. A.; Chopra, K.; Bradley, K. A.; Cohen, Y.; Harthorn, B. H.; Hoek, E. M.; Holden, P.; Keller, A. A.; Lenihan, H. S.; Nisbet, R. M.; Nel, A. E., The University of California Center for the Environmental Implications of Nanotechnology, Environ Sci Technol, 2009, 43 (17), 6453-7.
Maldonado-Arocho, F. J.; Bradley, K. A., Anthrax edema toxin induces maturation of dendritic cells and enhances chemotaxis towards macrophage inflammatory protein 3beta, Infect Immun, 2009, 77 (5), 2036-42.
Averette, K. M.; Pratt, M. R.; Yang, Y.; Bassilian, S.; Whitelegge, J. P.; Loo, J. A.; Muir, T. W.; Bradley, K. A., Anthrax lethal toxin induced lysosomal membrane permeabilization and cytosolic cathepsin release is Nlrp1b/Nalp1b-dependent, PLoS ONE, 2009, 4 (11), e7913.
Liong, Monty; France, Bryan; Bradley, Kenneth A.; Zink, Jeffrey I., Antimicrobial Activity of Silver Nanocrystals Encapsulated in Mesoporous Silica Nanoparticles, Advanced Materials, 2009, 21 (17), 1684-1689.
Lehrer, R. I.; Jung, G.; Ruchala, P.; Wang, W.; Micewicz, E. D.; Waring, A. J.; Gillespie, E. J.; Bradley, K. A.; Ratner, A. J.; Rest, R. F.; Lu, W., Human alpha-defensins inhibit hemolysis mediated by cholesterol-dependent cytolysins, Infect Immun, 2009, 77 (9), 4028-40.
Poulos, J. L.; Jeon, T. J.; Damoiseaux, R.; Gillespie, E. J.; Bradley, K. A.; Schmidt, J. J., Ion channel and toxin measurement using a high throughput lipid membrane platform, Biosens Bioelectron, 2009, 24 (6), 1806-10.
Lee, S.; Deng, H.; Yu, F.; Melega, W. P.; Damoiseaux, R.; Bradley, K. A.; Sun, R., Regulation of Kaposi's sarcoma-associated herpesvirus reactivation by dopamine receptor-mediated signaling pathways, J Acquir Immune Defic Syndr, 2008, 48 (5), 531-40.
Cote, C. K.; DiMezzo, T. L.; Banks, D. J.; France, B.; Bradley, K. A.; Welkos, S. L., Early interactions between fully virulent Bacillus anthracis and macrophages that influence the balance between spore clearance and development of a lethal infection, Microbes Infect, 2008, 10 (6), 613-9.
Sanchez, A. M.; Thomas, D.; Gillespie, E. J.; Damoiseaux, R.; Rogers, J.; Saxe, J. P.; Huang, J.; Manchester, M.; Bradley, K. A., Amiodarone and bepridil inhibit anthrax toxin entry into host cells, Antimicrob Agents Chemother, 2007, 51 (7), 2403-11.
Nassanian, H.; Sanchez, A. M.; Lo, A.; Bradley, K. A.; Lee, B., Efficient construction of an inverted minimal H1 promoter driven siRNA expression cassette: facilitation of promoter and siRNA sequence exchange, PLoS ONE, 2007, 2 (1), e767.
Banks, D. J.; Bradley, K. A., SILENCE: a new forward genetic technology, Nat Methods, 2007, 4 (1), 51-3.
Christman, K. L.; Requa, M. V.; Enriquez-Rios, V. D.; Ward, S. C.; Bradley, K. A.; Turner, K. L.; Maynard, H. D., Submicron streptavidin patterns for protein assembly, Langmuir, 2006, 22 (17), 7444-50.
Wang, W.; Mulakala, C.; Ward, S. C.; Jung, G.; Luong, H.; Pham, D.; Waring, A. J.; Kaznessis, Y.; Lu, W.; Bradley, K. A.; Lehrer, R. I., Retrocyclins kill bacilli and germinating spores of Bacillus anthracis and inactivate anthrax lethal toxin, J Biol Chem, 2006.
Maldonado-Arocho, F. J.; Fulcher, J. A.; Lee, B.; Bradley, K. A., Anthrax oedema toxin induces anthrax toxin receptor expression in monocyte-derived cells, Mol Microbiol, 2006, 61 (2), 324-37.
Salles,, II; Voth, D. E.; Ward, S. C.; Averette, K. M.; Tweten, R. K.; Bradley, K. A.; Ballard, J. D., Cytotoxic activity of Bacillus anthracis protective antigen observed in a macrophage cell line overexpressing ANTXR1, Cell Microbiol, 2006, 8 (8), 1272-81.
Ding, Z.; Bradley, K. A.; Amin Arnaout, M.; Xiong, J. P., Expression and purification of functional human anthrax toxin receptor (ATR/TEM8) binding domain from Escherichia coli, Protein Expr Purif, 2006, 49 (1), 121-8.
Banks, D. J.; Ward, S. C.; Bradley, K. A., New insights into the functions of anthrax toxin, Expert Rev Mol Med, 2006, 8 (7), 1-18.
Banks, D. J.; Barnajian, M.; Maldonado-Arocho, F. J.; Sanchez, A. M.; Bradley, K. A., Anthrax toxin receptor 2 mediates Bacillus anthracis killing of macrophages following spore challenge, Cell Microbiol, 2005, 7 (8), 1173-85.
Bruce, J. W.; Bradley, K. A.; Ahlquist, P.; Young, J. A., Isolation of cell lines that show novel, murine leukemia virus-specific blocks to early steps of retroviral replication, J Virol, 2005, 79 (20), 12969-78.
Sanchez, A. M.; Bradley, K. A., Anthrax toxin: can a little be a good thing?, Trends Microbiol, 2004, 12 (4), 143-5.
Bradley, K. A.; Mogridge, J.; Jonah, G.; Rainey, A.; Batty, S.; Young, J. A., Binding of anthrax toxin to its receptor is similar to alpha integrin-ligand interactions, J Biol Chem, 2003, 278 (49), 49342-7.
Scobie, H. M.; Rainey, G. J.; Bradley, K. A.; Young, J. A., Human capillary morphogenesis protein 2 functions as an anthrax toxin receptor, Proc Natl Acad Sci U S A, 2003, 100 (9), 5170-4.
Bradley, K. A.; Young, J. A., Anthrax toxin receptor proteins, Biochem Pharmacol, 2003, 65 (3), 309-14.
Bradley, K. A.; Mogridge, J.; Mourez, M.; Collier, R. J.; Young, J. A., Identification of the cellular receptor for anthrax toxin, Nature, 2001, 414 (6860), 225-9.